We analyzed urine and hair specimens from 120 workers undergoing second-level testing between 2009 and 2012. As regards urine samples, 71.2% of workers were always negative, 23.9% tested positive at least once for cannabinoids, and 2.5% for cocaine. Hair analyses produced surprising results: 61.9% of hair samples tested negative, only 6.2% tested positive for cannabinoids, whereas 28.8% tested positive for cocaine. These findings confirm that second-level surveillance of WDT, which includes hair analysis, is very effective because it highlightd drug intake – sometimes heavy – that cannot be revealed through a few urine analyses.

A double-blind placebo-controlled study (n = 10) was performed. Two subsequent doses of Δ⁹-tetrahydrocannabinol (THC) were administered using a Volcano vapourizer. Oral fluid (OF) screenings were positive just after smoking; however, sensitivity rapidly decreased within 1.5 h. The collected amount of OF diminished after smoking THC. Chronic cannabis users have high residual THC concentrations in their OF. Confirmation cut-offs should be set according to the aim of detecting recent drug use or establishing zero tolerance when applied to roadside drug testing.

More than 25 STS-135 metabolites were identified as oxidized, dealkylated or deflourinated, some of them further glucuronidated. Half-life and intrinsic clearance suggested a highly metabolized drug.

Sixteen phase-I metabolites of 5F-AKB-48 were identified in a human urine sample using LC-HRAM-MS, and compared to the metabolites generated from human liver microsomal incubations. The results may be used in clinical and forensic screening.

This study aimed to investigate the degration behavior and physicochemical stability of Tenofovir (TFV) using a liquid chromatography coupled mass spectrometry and solid state X-ray diffraction analyses. TFV showed degradation under under acidic and alkaline hydrolytic conditions. The structure of degradation products has been proposed. No significant degradation was observed at pH 4.5 (normal CV fluid pH), oxidative, accelerated and thermal stress conditions. The shelf life of TFV at room temperature was found to be 23 months as calculated by using Arrhenius equation plot.

LC-MS/MS multi-analyte method for target screening of benzodiazepines, antidepresssants, neuroleptics, and others in different biosamples after one simple extraction approach is presented. Validation results required for qualitative methods are compared between eight different biosamples.

South American ‘crack’ can be distinguished from US domestically produced crack on the basis of occluded solvent profiles. In addition, analysis of domestically produced crack indicates the solvents that were used for cocaine hydrochloride processing in South America.

The exact structures of two glucuronides of 6β-OH-androsterone and 6β-OH-etiocholanolone, previously shown to be highly resistant against enzymatic hydrolysis, were elucidated. Their structural elucidation was achieved after synthesis of four possible diasteromers, characterization by NMR spectroscopy and comparison of their UHPLC-MS/MS properties to the ones obtained in urine sample after testosterone administration. The results of this study provide the necessary tools for the development of analytical methods in order to evaluate these metabolites as biomarkers for testosterone misuse.