Front Cover

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Front cover: Large-scale multiplex absolute protein quantification of drug-metabolizing enzymes and transporters in human intestine, liver, and kidney microsomes by SWATH-MS: Comparison with MRM/SRM and HR-MRM/PRM

  • First Published: 20 August 2016
Description unavailable

DOI: 10.1002/pmic.201500433

Metabolizing enzymes and transporters are key molecules that function cooperatively to regulate the absorption, metabolism and excretion of medicinal drugs. Absolute protein levels of 30 metabolizing enzymes and 107 transporters were simultaneously quantified in microsomes of human liver, kidney and intestine by means of SWATH-MS with stable isotope-labeled peptides, and the results were compared with those obtained by MRM/SRM and high resolution-MRM/PRM. For more details, see the article by Kenji Nakamura et al. on pages 2106–2117.

Inside Front Cover

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Inside Front Cover: Multi-mode acquisition (MMA): An MS/MS acquisition strategy for maximizing selectivity, specificity and sensitivity of DIA product ion spectra

  • First Published: 20 August 2016
Description unavailable

DOI: 10.1002/pmic.201500492

There is a fixed amount of m/z sufficient space in an MS/MS spectrum. The number of precursors that can accurately identified is a function of the pre-fragmentation resolving powers (m/z, chromatographic and drift). Precursor ion flux is not uniform across time, drift or m/z. This variability necessitates the width of the mass isolation window (MIW) to be dynamic. The described MMA workflow uses models and prior knowledge to dynamically set the MIW over the gradient elution. For more details, see the article by Brad J. Williams et al. on pages 2284–2301.

Editorial Board

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Editorial Board: Proteomics 15–16'16

  • First Published: 20 August 2016

Contents

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Contents: Proteomics 15–16'16

  • First Published: 20 August 2016

Applications in targeted proteomics

Reviews and comparisons in targeted proteomics

Developments in acquisition and data analysis