Volume 31, Issue 11 p. 937-950
Research Article

Rapid and sensitive determination of multiple endocrine-disrupting chemicals by ultrasound-assisted in situ derivatization dispersive liquid–liquid microextraction coupled with ultra-high-performance liquid chromatography/tandem mass spectrometry

Na Wei

Na Wei

Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165 Shandong, P.R. China

These authors contributed equally to this work.Search for more papers by this author
Zhenjia Zheng

Zhenjia Zheng

College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Taian, 271018 Shandong, P.R. China

These authors contributed equally to this work.Search for more papers by this author
Yuhua Wang

Yuhua Wang

Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165 Shandong, P.R. China

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Yanduo Tao

Yanduo Tao

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, 810001 Qinghai, P.R. China

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Yun Shao

Yun Shao

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, 810001 Qinghai, P.R. China

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Shuyun Zhu

Corresponding Author

Shuyun Zhu

Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165 Shandong, P.R. China

Correspondence to: X.-E. Zhao, S. Y. Zhu and J. M. You, Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, Shandong, P.R. China.

E-mail: [email protected]; [email protected]; [email protected]

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Jinmao You

Corresponding Author

Jinmao You

Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165 Shandong, P.R. China

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, 810001 Qinghai, P.R. China

Correspondence to: X.-E. Zhao, S. Y. Zhu and J. M. You, Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, Shandong, P.R. China.

E-mail: [email protected]; [email protected]; [email protected]

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Xian-En Zhao

Corresponding Author

Xian-En Zhao

Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165 Shandong, P.R. China

Correspondence to: X.-E. Zhao, S. Y. Zhu and J. M. You, Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, Shandong, P.R. China.

E-mail: [email protected]; [email protected]; [email protected]

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First published: 31 March 2017
Citations: 25

Abstract

Rationale

Endocrine-disrupting chemicals (EDCs) in environment samples and food stuffs are an increasing serious public health issue due to their potency to interfere and deregulate several aspects of the endocrine system. Because of their extremely low abundance, it remains a challenging task to develop a sensitive detection method.

Methods

4'-Carbonyl chloride rosamine (CCR) was used as a derivatization reagent for EDCs for the first time. A new ultrasound-assisted in situ derivatization/dispersive liquid-liquid microextraction (UA-DLLME with in situ derivatization) method for multiple EDCs including five estrogens, two alkylphenols, eight bisphenols, seven parabens and triclosan coupled with ultra-high-performance liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS) has been developed and validated.

Results

The ionization efficiency of EDCs was greatly enhanced through the introduction of a permanent charged moiety of CCR into the derivatives during electrospray ionization (ESI)-MS analysis. The main variables potentially affecting the UA-DLLME with in situ derivatization process are optimized. The recoveries and matrix effects of 23 EDCs for the spiking samples were in the range of 83.0–116.0% and 85.8–114.6%, respectively. Good method reproducibility was achieved.

Conclusions

The limits of detection (LODs) for 23 EDCs were 0.05–0.40 ng/L and 0.03–0.25 ng/g (dry weight, d.w.) for environment samples and food stuffs, respectively. The proposed method has been demonstrated to be suitable for simultaneous determination of multiple EDCs in real samples with high sensitivity, speediness, and good sample clean-up ability. Copyright © 2017 John Wiley & Sons, Ltd.