Volume 34, Issue 24 p. 3460-3469
Review

Separation strategies for untargeted metabolomics

Gary J. Patti

Corresponding Author

Gary J. Patti

Departments of Chemistry, Genetics, and Medicine Washington University, School of Medicine, St. Louis, MO, USA

Departments of Chemistry, Genetics, and Medicine Washington University, School of Medicine, 660 S. Euclid Ave., Box 8232, St. Louis, MO 63110, USA Fax: +1-314-362-8756Search for more papers by this author
First published: 04 October 2011
Citations: 105

Abstract

Metabolomics has rapidly become a profiling technique of choice for biomarker elucidation and molecular diagnostics in addition to studies focused on understanding disease pathogenesis. Key to the success of metabolomics in these areas has been the techniques to separate and analyze the chemically diverse group of compounds comprising the metabolome by using global and untargeted approaches. Untargeted metabolomic efforts have the goal of examining as many metabolites as possible simultaneously and most frequently use an LC/MS-based approach. Here, the importance of LC in an untargeted metabolomic workflow is outlined and separation strategies for optimization are reviewed within the context of these criteria.