Volume 9, Issue 3 p. 128-131
Original Paper

Isoelectric focusing of catalase from acatalasemic mouse and human blood, and cultured human skin fibroblasts

Dr. Masana Ogata

Corresponding Author

Dr. Masana Ogata

Department of Public Health, Okayama University Medical School, Okayama

Department of Public Health, Okayama University Medical School, Shikata-Cho, Okayama 700, Japan===Search for more papers by this author
Yukinori Satoh

Yukinori Satoh

Department of Public Health, Okayama University Medical School, Okayama

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First published: 1988
Citations: 10

Abstract

Hemolysates of normal, heterozygous hypocatalasemic and acatalasemic mice and of Japanese acatalasemic subjects were separated into three fractions, A, B and C, by DEAE-cellulose column chromatography, and pI values of A, B and C fractions were determined by isoelectric focusing. The pI value of catalase in the A, B and C fractions increased in the order of normal, hypocatalasemic and acatalasemic mouse blood. The results obtained from Japanese acatalasemic blood samples showed that the pI values of catalase in the A, B and C fractions were similar to those in normal blood. Catalase in Japanese acatalasemic cultured skin fibroblasts was also analyzed by isoelectric focusing. The pI values of catalase in the extract from the cultured skin acatalasemic fibroblasts was similar to that in normal fibroblasts.