Volume 34, Issue 12 p. 1928-1935
Research Article

Electrogenerated Chemiluminescent Detection of Polyamines on a Microfluidic Device Using Micromolded Carbon Paste Microelectrodes

Erin M. Gross

Corresponding Author

Erin M. Gross

Creighton University, Department of Chemistry and Biochemistry, Omaha, Nebraska, USA

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Emily R. Lowry

Emily R. Lowry

Creighton University, Department of Chemistry and Biochemistry, Omaha, Nebraska, USA

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Leah V. Schaffer

Leah V. Schaffer

University of California San Diego, Department of Medicine, San Diego, California, USA

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Charles S. Henry

Charles S. Henry

Colorado State Department of Chemistry, Colorado State University, Fort Collins, Colorado, USA

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First published: 02 February 2022
Citations: 3

Abstract

A simple, sensitive, and rapid electrochemiluminescent (ECL) method was developed for the detection of several polyamines on a microfluidic chip. Rapid prototyping of poly(dimethylsiloxane) was used to fabricate flow channels and carbon paste microelectrodes. These devices are easily fabricated and amenable to ECL detection due to their planar and optically transparent nature. The ECL reaction between tris(2,2′-bipyridyl)ruthenium(II) and amine-containing co-reactants was used to generate the ECL signal. Experimental parameters were optimized and low micromolar limits of detection (S/N=3) were determined for four different polyamines. The method was successfully used to determine the concentration of spermine in milk samples.

Data Availability Statement

The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.