Volume 38, Issue 6 p. 672-679
RESEARCH ARTICLE

Green micellar spectrofluorimetric determination of α-mangostin found in herbal products as antioxidant and other biological activities beneficial to human health

Hesham Salem

Corresponding Author

Hesham Salem

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Deraya University, New Minia, Egypt

Correspondence

Hesham Salem, Pharmaceutical Chemistry Department, Faculty of Pharmacy, Deraya University, New Minia, Egypt.

Email: [email protected]; [email protected]

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Dina Z. Mazen

Dina Z. Mazen

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Deraya University, New Minia, Egypt

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Nadeen Emad

Nadeen Emad

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Deraya University, New Minia, Egypt

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Hossam Hassan

Hossam Hassan

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Deraya University, New Minia, Egypt

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Bahaa Omran

Bahaa Omran

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Deraya University, New Minia, Egypt

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Mahmoud A. Abdelmajed

Mahmoud A. Abdelmajed

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Deraya University, New Minia, Egypt

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First published: 19 April 2023

Funding information: Open access funding provided by The Science, Technology & Innovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge Bank (EKB). No funding was received for this work.

Abstract

Pharmaceutical product quality control (QC) needs quick, sensitive and economical procedures to deliver high throughput at low cost, which is the key factor considered by such economic facilities. To lessen the risky effects of research laboratories, researchers must take into account the ecological impacts. α-Mangostin (MAG) exhibit anti-inflammatory, antioxidant, anticancer, anti-allergic, antibacterial, antifungal, antiviral and antimalarial activities. Based on the spectrofluorimetric approach, a novel straightforward, sensitive and environmentally friendly method for MAG determination was developed and validated. Many variables were investigated to improve MAG native fluorescence, including solvent type, buffers, pH and additional surfactants. The best MAG fluorescence sensitivity was found in Britton–Robinson buffer (pH 4) at 450 nm after irradiation at 350 nm in the concentration range of 5–50 ng ml−1. The technique was successfully used to determine the presence of MAG in both its approved dose forms and in samples of spiked human plasma, as per FDA standards for validation. According to their evaluation on two recent greenness criteria (GAPI [Green Analytical Procedure Index] and AGREE [Analytical GREEnness]), the suggested approach has been shown to be environmentally beneficial because it normally uses biodegradable chemicals in solvent-free aqueous phases.

CONFLICT OF INTEREST STATEMENT

The authors declare that they have no competing financial interests.

DATA AVAILABILITY STATEMENT

Data are available on request.