Volume 38, Issue 4 p. 437-449
RESEARCH ARTICLE

Newly synthesized Fe-doped CDs for colorimetric and fluorometric nanozyme-based levodopa sensing

Haval Aziz Ahmad

Haval Aziz Ahmad

Department of Chemistry-College of Science, University of Raparin, Sulaymaniyah, Iraq

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Rebwar Omar Hassan

Corresponding Author

Rebwar Omar Hassan

Department of Chemistry-College of Science, Salahaddin University, Erbil, Kurdistan Region, Iraq

Correspondence

Rebwar Omar Hassan, Department of Chemistry-College of Science, Salahaddin University, Erbil, Kurdistan Region 44001, Iraq.

Email: [email protected]

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First published: 16 February 2023
Citations: 2

Abstract

A simple single-pot hydrothermal method was used to fabricate a Fe, N, and S co-doped carbon dots (Fe-CDs) nanozyme using ferric chloride and sunset yellow as precursors. The fabricated Fe-CDs exhibited intense green fluorescence at 460 nm with excitation-independent properties and a high quantum yield of 40.23%. This nanozyme mimics peroxidase by catalyzing the oxidation of tetramethylbenzidine (TMB) by H2O2 to yield a blue-coloured TMBox product at 652 nm. Dual detection methods were established for determining levodopa (l-dopa) by taking advantage of the high nanozyme activity and the distinct fluorescence aspect. Both determination methods are based on the oxidation of l-dopa by H2O2 in the presence of Fe-CDs and fading of the blue colour of the TMBox. The colorimetric method monitors the amount of colour fading of TMBox. In the fluorometric method, the formed blue TMBox absorbs the emission light of the Fe-CDs; when l-dopa is present, this effect decreases and the intensity of the emission light increases. The nanozyme-based detection procedures exhibit good linearity in the ranges 2.17 × 10−3 to 34.78 × 10−3 mM [limit of detection (LOD) = 0.84 × 10−3 mM] and 0.85 × 103 to 16.95 × 103 nM (LOD = 0.102 × 103 nM) for colorimetric and fluorometric methods, respectively.

CONFLICT OF INTEREST

None of the writers of this paper has any conflicts of interest.

DATA AVAILABILITY STATEMENT

Original contributions reflected in this work can be obtained from the article or supplementary material.