Volume 18, Issue 9 1700447
Research Article

Anton Chekhov and Robert Koch Cheek to Cheek: A Proteomic Study

Alfonsina D'Amato

Alfonsina D'Amato

Quadram Institute Bioscience, Norwich Research Park, Norwich, England

Search for more papers by this author
Gleb Zilberstein

Gleb Zilberstein

Spectrophon Ltd, Rehovot, Israel

Search for more papers by this author
Svetlana Zilberstein

Svetlana Zilberstein

Spectrophon Ltd, Rehovot, Israel

Search for more papers by this author
Mikhail Ivanovich Golovan

Mikhail Ivanovich Golovan

State Literary and Memorial Museum–Reserve A.P. Chekhov, Melikhovo, Russia

Search for more papers by this author
Anastasiya Anatolyevna Zhuravleva

Anastasiya Anatolyevna Zhuravleva

State Literary and Memorial Museum–Reserve A.P. Chekhov, Melikhovo, Russia

Search for more papers by this author
Pier Giorgio Righetti

Corresponding Author

Pier Giorgio Righetti

Department of Chemistry, Materials and Chemical Engineering ‘‘Giulio Natta’’, Politecnico di Milano, Milano, Italy

Correspondence: Professor Pier Giorgio Righetti, Department of Chemistry, Materials and Chemical Engineering “Giulio Natta,” Politecnico di Milano, 20131 Milan, Italy

E-mail: [email protected]

Search for more papers by this author
First published: 09 March 2018
Citations: 16

Abstract

Five different letters and post cards as well as the shirt worn by Anton Chekhov on his death bed, stored in the State Literary-Memorial Museum-Reserve A. P. Chekhov Melikhovo (nearby Moscow), have been analyzed by applying EVA (an ethyl vinyl acetate foil studded with crushed strong anion and cation exchangers and with C8 resins) diskettes to these surfaces. Three different eluates (under acidic and basic conditions and with acetonitrile) were analyzed by high resolution mass spectrometry. The environmental microbiota present on samples and the Mycobacterium tuberculosis strain were described by a meta-proteomics approach. Eight identified M. tuberculosis proteins confirmed the presence of the bacterium and the cause of Chekhov's death, in addition to several sequenced peptides belonging to other bacterial species. The human plasma proteins and human keratins, detected on a tiny blood spot on the shirt, demonstrated the power of the combined approach.

Conflict of Interest

The authors declare no conflict of interest.