Volume 13, Issue 9 p. 1513-1518
Dataset Brief

A two-dimensional proteome map of the aflatoxigenic fungus Aspergillus flavus

Olga Pechanova

Olga Pechanova

Mississippi State Chemical Laboratory, Mississippi State University, Mississippi State, MS, USA

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Tibor Pechan

Corresponding Author

Tibor Pechan

Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, MS, USA

Correspondence: Dr. Tibor Pechan, Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, MS 39762, USA

E-mail:[email protected]

Fax: +1-662-325-9264

Additional corresponding author: Dr. Ashli E. Brown,

E-mail: [email protected]

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Jose M. Rodriguez

Jose M. Rodriguez

Mississippi State Chemical Laboratory, Mississippi State University, Mississippi State, MS, USA

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W. Paul Williams

W. Paul Williams

US Department of Agriculture, Agricultural Research Services, Corn Host Plant Resistance Research Unit, Mississippi State, MS, USA

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Ashli E. Brown

Corresponding Author

Ashli E. Brown

Department of Biochemistry, Molecular Biology, Entomology, and Pant Pathology, Mississippi State University, Mississippi State, MS, USA

Correspondence: Dr. Tibor Pechan, Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, MS 39762, USA

E-mail:[email protected]

Fax: +1-662-325-9264

Additional corresponding author: Dr. Ashli E. Brown,

E-mail: [email protected]

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First published: 04 March 2013
Citations: 22

Abstract

The filamentous fungus Aspergillus flavus is an opportunistic soil-borne pathogen that produces aflatoxins, the most potent naturally occurring carcinogenic compounds known. This work represents the first gel-based profiling analysis of A. flavus proteome and establishes a 2D proteome map. Using 2DE and MALDI-TOF-MS/MS, we identified 538 mycelial proteins of the aflatoxigenic strain NRRL 3357, the majority of which were functionally annotated as related to various cellular metabolic and biosynthetic processes. Additionally, a few enzymes from the aflatoxin synthesis pathway were also identified.