Volume 11, Issue 12 p. 2459-2475
Research Article

Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy

Pyong-Gon Moon

Pyong-Gon Moon

Department of Molecular Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea

Cell and Matrix Biology Research Institute, School of Medicine, Kyungpook National University, Daegu, Republic of Korea

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Jeong-Eun Lee

Jeong-Eun Lee

Department of Molecular Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea

Cell and Matrix Biology Research Institute, School of Medicine, Kyungpook National University, Daegu, Republic of Korea

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Sungyong You

Sungyong You

School of Interdisciplinary Bioscience and Bioengineering and Department of Chemical Eng., POSTECH, Pohang, Republic of Korea

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Taek-Kyun Kim

Taek-Kyun Kim

School of Interdisciplinary Bioscience and Bioengineering and Department of Chemical Eng., POSTECH, Pohang, Republic of Korea

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Ji-Hoon Cho

Ji-Hoon Cho

School of Interdisciplinary Bioscience and Bioengineering and Department of Chemical Eng., POSTECH, Pohang, Republic of Korea

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In-San Kim

In-San Kim

Cell and Matrix Biology Research Institute, School of Medicine, Kyungpook National University, Daegu, Republic of Korea

Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea

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Tae-Hwan Kwon

Tae-Hwan Kwon

Cell and Matrix Biology Research Institute, School of Medicine, Kyungpook National University, Daegu, Republic of Korea

Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea

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Chan-Duck Kim

Chan-Duck Kim

Department of Internal Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea

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Sun-Hee Park

Sun-Hee Park

Department of Internal Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea

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Daehee Hwang

Daehee Hwang

School of Interdisciplinary Bioscience and Bioengineering and Department of Chemical Eng., POSTECH, Pohang, Republic of Korea

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Yong-Lim Kim

Yong-Lim Kim

Department of Internal Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea

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Moon-Chang Baek

Corresponding Author

Moon-Chang Baek

Department of Molecular Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea

Cell and Matrix Biology Research Institute, School of Medicine, Kyungpook National University, Daegu, Republic of Korea

Department of Molecular Medicine, School of Medicine, Kyungpook National University, 101 Dongin-dong 2 Ga, Jung-gu, Daegu, 700-422, Republic of Korea Fax: +82-53-426-4944===Search for more papers by this author
First published: 29 March 2011
Citations: 201

Colour Online: See the article online to view Fig. 8 in colour.

Abstract

To identify biomarker candidates associated with early IgA nephropathy (IgAN) and thin basement membrane nephropathy (TBMN), the most common causes presenting isolated hematuria in childhood, a proteomic approach of urinary exosomes from early IgAN and TBMN patients was introduced. The proteomic results from the patients were compared with a normal group to understand the pathophysiological processes associated with these diseases at the protein level. The urinary exosomes, which reflect pathophysiological processes, collected from three groups of young adults (early IgAN, TBMN, and normal) were trypsin-digested using a gel-assisted protocol, and quantified by label-free LC-MS/MS, using an MSE mode. A total of 1877 urinary exosome proteins, including cytoplasmic, membrane, and vesicle trafficking proteins, were identified. Among the differentially expressed proteins, four proteins (aminopeptidase N, vasorin precursor, α-1-antitrypsin, and ceruloplasmin) were selected as biomarker candidates to differentiate early IgAN from TBMN. We confirmed the protein levels of the four biomarker candidates by semi-quantitative immunoblot analysis in urinary exosomes independently prepared from other patients, including older adult groups. Further clinical studies are needed to investigate the diagnostic and prognostic value of these urinary markers for early IgAN and TBMN. Taken together, this study showed the possibility of identifying biomarker candidates for human urinary diseases using urinary exosomes and might help to understand the pathophysiology of early IgAN and TBMN at the protein level.