Proteome analysis of Schizosaccharomyces pombe by two-dimensional gel electrophoresis and mass spectrometry
Kyung-Hoon Hwang
Universität des Saarlandes, FR 8.3 Biochemie, Saarbrücken, Germany
These authors contributed equally to this work.
Search for more papers by this authorChristine Carapito
Laboratoire de Spectrométrie de Masse Bio-Organique, UMR 7512 CNRS/ULP, ECPM, Strasbourg, France
These authors contributed equally to this work.
Search for more papers by this authorSusanne Böhmer
Universität des Saarlandes, FR 8.3 Biochemie, Saarbrücken, Germany
Search for more papers by this authorEmmanuelle Leize
Laboratoire de Spectrométrie de Masse Bio-Organique, UMR 7512 CNRS/ULP, ECPM, Strasbourg, France
Search for more papers by this authorAlain Van Dorsselaer
Laboratoire de Spectrométrie de Masse Bio-Organique, UMR 7512 CNRS/ULP, ECPM, Strasbourg, France
Search for more papers by this authorCorresponding Author
Rita Bernhardt Dr.
Universität des Saarlandes, FR 8.3 Biochemie, Saarbrücken, Germany
Universität des Saarlandes, FR 8.3 Biochemie, P.O. Box 151150, D-66041 Saarbrücken, Germany Fax: +49-681-302-4739===Search for more papers by this authorKyung-Hoon Hwang
Universität des Saarlandes, FR 8.3 Biochemie, Saarbrücken, Germany
These authors contributed equally to this work.
Search for more papers by this authorChristine Carapito
Laboratoire de Spectrométrie de Masse Bio-Organique, UMR 7512 CNRS/ULP, ECPM, Strasbourg, France
These authors contributed equally to this work.
Search for more papers by this authorSusanne Böhmer
Universität des Saarlandes, FR 8.3 Biochemie, Saarbrücken, Germany
Search for more papers by this authorEmmanuelle Leize
Laboratoire de Spectrométrie de Masse Bio-Organique, UMR 7512 CNRS/ULP, ECPM, Strasbourg, France
Search for more papers by this authorAlain Van Dorsselaer
Laboratoire de Spectrométrie de Masse Bio-Organique, UMR 7512 CNRS/ULP, ECPM, Strasbourg, France
Search for more papers by this authorCorresponding Author
Rita Bernhardt Dr.
Universität des Saarlandes, FR 8.3 Biochemie, Saarbrücken, Germany
Universität des Saarlandes, FR 8.3 Biochemie, P.O. Box 151150, D-66041 Saarbrücken, Germany Fax: +49-681-302-4739===Search for more papers by this authorAbstract
The fission yeast Schizosaccharomyces pombe (S. pombe) is a unicellular eukaryote and contains many genes and regulatory mechanisms that are close to those of mammals. In this study, we performed a global proteomic analysis of the fission yeast S. pombe wild type h−S L 972 proteome. More than 1500 protein spots were visualized on silver stained 2-D gels in the 3–10 pI range with a high resolution and high reproducibility. Protein identification was carried out by MALDI-TOF-MS and/or nanoLC-MS/MS. Advantage of the complementarity of these two MS approaches was used to enhance the identification quality. So far, 364 proteins (representing 157 different proteins) have been identified. We report here the identification of 117 new proteins on our 2-D reference map of this yeast compared to the first reference map. Of these identified proteins, 40.1% were involved in metabolism. The present work provides a very useful tool for all studies relying on S. pombe as a model organism and is a considerable complement to the first reference map of S. pombe published recently by Sun and coworkers (Sun, N., Jang, J., Lee, S., Kim, S. et al.., Proteomics 2005, 5, 1574–1579).
Supporting Information
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