Volume 46, Issue 13 2201057
RESEARCH ARTICLE

Synthesis of core-shell material rich in carboxyl groups and its application in the selective adsorption of cationic peptides

Shadi Ali Hassen Ahmed

Shadi Ali Hassen Ahmed

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, P. R. China

College of Pharmaceutical Sciences, Hangzhou Institute of Innovative Medicine, Zhejiang University, Hangzhou, P. R. China

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YeTong Yang

YeTong Yang

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, P. R. China

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Shuming Li

Shuming Li

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, P. R. China

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Haobin Shi

Haobin Shi

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, P. R. China

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Linghui Qian

Linghui Qian

College of Pharmaceutical Sciences, Hangzhou Institute of Innovative Medicine, Zhejiang University, Hangzhou, P. R. China

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Jing Zhang

Corresponding Author

Jing Zhang

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, P. R. China

Correspondence

Jing Zhang, Shaanxi Normal University, No.620, Xichangan Road Changan District, Xi'an, Shaanxi. 710119, P. R. China.

Email: [email protected]

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First published: 09 April 2023

Shadi Ali Hassen Ahmed and YeTong Yang contributed equally to this study.

Abstract

The ability to extract peptides and proteins from biological samples with excellent reusability, high adsorption capacity, and great selectivity is essential in scientific research and medical applications. Inspired by the advantages of core-shell materials, we fabricated a core-shell material using amino-functionalized silica as the core. Benzene-1,3,5-tricarbaldehyde and 3,5-diaminobenzoic acid were used as model organic ligands to construct a shell coating by alternately reacting the two monomers on the surface of silica microspheres. The resultant material featured an outstanding capability for the adsorption of cationic peptides, most likely owing to its porous structure, a large number of carboxylic functional groups, and low mass-transfer resistance. The maximum saturated adsorption capacity reached 833.3 mg/g and the adsorption process took only 20 min. Under optimized adsorption conditions, the core-shell material was used to selectively adsorb cationic peptides from the tryptic digestive solution of lysozyme and bovine serum albumin, Specifically, the analysis results showed seven cationic peptides in the eluate and twenty anionic peptides in the supernatant, which indicates the efficient trap of most cationic peptides in the digestive solution.

CONFLICT OF INTEREST STATEMENT

The authors declare no conflict of interest.

DATA AVAILABILITY STATEMENT

The data that support the findings of this study are available in the supplementary material of this article.