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Monolithic stationary phases with a longitudinal gradient of porosity

Corresponding Author

Jiří Urban

[email protected]

Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic

Current address: Dr. Jiří Urban, Department of Chemistry, Faculty of Science, Masaryk University, 625 00 Brno, Czech Republic.

Email: [email protected]

Correspondence

Dr. Jiří Urban, Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice, Czech Republic, CZ-532 10.

Fax: +420466037068

Emails: [email protected];

[email protected]

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Tomáš Hájek,

Tomáš Hájek

Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic

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Frantisek Svec,

Frantisek Svec

The Molecular Foundry, E.O. Lawrence Berkeley National Laboratory, Berkeley, CA, USA

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Jiří Urban,

Corresponding Author

Jiří Urban

[email protected]

Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic

Current address: Dr. Jiří Urban, Department of Chemistry, Faculty of Science, Masaryk University, 625 00 Brno, Czech Republic.

Email: [email protected]

Correspondence

Dr. Jiří Urban, Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice, Czech Republic, CZ-532 10.

Fax: +420466037068

Emails: [email protected];

[email protected]

Search for more papers by this author

Tomáš Hájek,

Tomáš Hájek

Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic

Search for more papers by this author

Frantisek Svec,

Frantisek Svec

The Molecular Foundry, E.O. Lawrence Berkeley National Laboratory, Berkeley, CA, USA

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First published: 22 February 2017

https://doi.org/10.1002/jssc.201700048

Citations: 10

Conflict of interest: The authors have declared no conflict of interest.

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Abstract

The duration of the hypercrosslinking reaction has been used to control the extent of small pores formation in polymer-based monolithic stationary phases. Segments of five columns hypercrosslinked for 30–360 min were coupled via zero-volume unions to prepare columns with segmented porosity gradients. The steepness of the porosity gradient affected column efficiency, mass transfer resistance, and separation of both small-molecule alkylbenzenes and high-molar-mass polystyrene standards. In addition, the segmented column with the steepest porosity gradient was prepared as a single column with a continuous porosity gradient. The steepness of porosity gradient in this type column was tuned. Compared to a completely hypercrosslinked column, the column with the shallower gradient produced comparable size-exclusion separation of polystyrene standards but allowed higher column permeability. The completely hypercrosslinked column and the column with porosity gradient were successfully coupled in online two-dimensional liquid chromatography of polymers.

Supporting Information

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Volume40, Issue8

April 2017

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Monolithic stationary phases with a longitudinal gradient of porosity

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Monolithic stationary phases with a longitudinal gradient of porosity

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