Volume 31, Issue 19 p. 3297-3308

Liquid Chromatography

Use of partially porous column as second dimension in comprehensive two-dimensional system for analysis of polyphenolic antioxidants

Paola Dugo,

Paola Dugo

[email protected]

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Francesco Cacciola,

Francesco Cacciola

Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, Messina, Italy

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Miguel Herrero,

Miguel Herrero

Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, Messina, Italy

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Paola Donato,

Paola Donato

Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, Messina, Italy

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Luigi Mondello,

Luigi Mondello

Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, Messina, Italy

Campus-Biomedico, Roma, Italy

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Paola Dugo,

Paola Dugo

[email protected]

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Francesco Cacciola,

Francesco Cacciola

Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, Messina, Italy

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Miguel Herrero,

Miguel Herrero

Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, Messina, Italy

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Paola Donato,

Paola Donato

Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, Messina, Italy

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Luigi Mondello,

Luigi Mondello

Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, Messina, Italy

Campus-Biomedico, Roma, Italy

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First published: 16 October 2008

https://doi.org/10.1002/jssc.200800281

Citations: 64

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Abstract

In the present work, a comprehensive LC system using a microbore HPLC column in the first dimension and a partially porous column in the second dimension was developed and applied to the separation of polyphenolic components in a red wine sample. The performance of the partially porous short column (3.0 cm) was compared to that of a monolithic column, of comparable dimensions. The results obtained demonstrated the possibility to use partially porous columns to obtain fast analyses, using high flow rates, under repetitive gradient conditions and with very brief reconditioning times. A conventional HPLC system was used since the backpressure generated by the shell-packed column, even at very high flow rates, was well within the operational limits. The use of an increased column temperature (60°C) allowed a further pressure-drop decrease, with no stationary phase degradation, or loss in column performance.

References

[1] Macheix, J. J., Fleuriet, A., Billot, J., Fruit Phenolics, CRC Press, Boca Raton FL 1990.
[2] Moure, A., Cruz, J. M., Franco, D., Dominguez, J. M. et al., Food Chem. 2001, 72, 145–171.
[3] Dugas, A. J. J., Castaneda-Acosta, J., Bonin, G. C., Price, K. L. et al., J. Nat. Prod. 2000, 63, 327–331.
[4] Ng, T. B., Liu, F., Wang, Z. T., Life Sci. 2000, 66, 709–723.
[5] Crespo, M. E., Galvez, J., Cruz, T., Ocete, M. A., Zarzuelo, A., Planta Med. 1999, 65, 651–653.
[6] Butterweck, V., Jurgenliemk, G., Nahrstedt, A., Winterhoff, H., Planta Med. 2000, 66, 3–6.
[7] Mouly, P., Gaydon, E. M., Auffrey, A., J. Chromatogr. A 1998, 800, 171–179.
[8] Cacciola, F., Jandera, P., Blahová, E., Mondello, L., J. Sep. Sci. 2006, 29, 2500–2513.
[9] Cacciola, F., Jandera, P., Mondello, L., J. Sep. Sci. 2007, 30, 462–474.
[10] Cacciola, F., Jandera, P., Hajdu, Z., Cesla, P., Mondello, L., J. Chromatogr. A 2007, 1149, 73–87.
[11] Jandera, P., Cesla, P., Hájek, T., Vohralík, G. et al., J. Chromatogr. A 2007, 1189, 207–220.
[12] Kivilompolo, M., Hyötyäinen, T., J. Chromatogr. A 2007, 1145, 155–164.
[13] Giddings, J. C., Anal. Chem. 1984, 56, 1258A–1270A.
[14] Giddings, J. C., HRC CC J. High Resolut. Chromatogr. Chromatogr. Commun. 1987, 10, 319–323.
[15] Jandera, P., J. Sep. Sci. 2006, 29, 1763–1783.
[16] Van der Horst, A., Schoenmakers, P. J., J. Chromatogr. A 2003, 1000, 693–709.
[17] Dugo, P., Favoino, O., Luppino, R., Dugo, G., Mondello, L., Anal. Chem. 2004, 76, 2525–2530.
[18] Jandera, P., Fischer, J., Lahovská, H., Novotná, K. et al., J. Chromatogr. A 2006, 1119, 3–10.
[19] Dugo, P., Kumm, T., Crupi, M. L., Cotroneo, A., Mondello, L., J. Chromatogr. A 2006, 1112, 269–275.
[20] Dugo, P., Škeriková, V., Kumm, T., Trozzi, A. et al., Anal. Chem. 2006, 78, 7743–7750.
[21] Dugo, P., Cacciola, F., Kumm, T., Dugo, G., Mondello, L., J. Chromatogr. A 2006, 1112, 269–275.
[22] Lubda, D., Cabrera, K., Kraas, W., Shafer, C., Cunningham, D., LC GC Eur. 2001, 14, 730–734.
[23] Stoll, D. R., Cohen, J. D., Carr, P. W., J. Chromatogr. A 2006, 1122, 123–137.
[24] Stoll, D. R., Li, X., Wang, X., Carr, P. W., J. Chromatogr. A 2007, 1168, 3–43.
[25] Way, W., The Reporter USA 2007, 25, 6–7.
[26] Way, W. K., The Reporter Europe, 2007, October, 28, 3–4.
[27] Giddings, J. C., Unified Separation Science, Wiley Interscience, New York 1991.
[28] Gritti, F., Cavazzini, A., Marchetti, N., Guiochon, G., J. Chromatogr. A 2007, 1157, 289–303.
[29] Gritti, F., Guiochon, G., J. Chromatogr. A 2007, 1166, 30–46.
[30] Marchetti, N., Cavazzini, A., Gritti, F., Guiochon, G., J. Chromatogr. A 2007, 1163, 203–211.
[31] Cavazzini, A., Gritti, F., Kaczmarski, K., Marchetti, N., Guiochon, G., Anal. Chem. 2007, 79, 5972–5979.
[32] Cunliffe, J. M., Maloney, T. D., J. Sep. Sci. 2007, 30, 3104–3109.
[33] La Torre, G. L., Saitta, M., Vilasi, F., Pellicanò, T., Dugo, G., Food Chem. 2006, 94, 640–650.
[34] Buiarelli, F., Cartoni, G., Coccioli, F., Levetsovitou, Z., J. Chromatogr. A 1995, 695, 229–235.
[35] Vinas, P., Lopez-Erroz, C., Marin-Hernandez, J. J., Hernandez-Cordoba, M., J. Chromatogr. A 2000, 871, 85–93.
[36] Revilla, E., Ryan, J. M., J. Chromatogr. A 2000, 881, 461–469.
[37] Rodriguez-Delgado, M. A., Malovaná, Pérez, J. P., Borse, T., García Montelongo, F. J., J. Chromatogr. A 2001, 912, 249–257.
[38] Frankel, E. N., Kanner, J., German, J. B., Parks, E., Kinsella, J. E., Lancet 1993, 341, 454–457.
[39] Teissedre, P. L., Frankel, E. N., Waterhouse, A. L., Peleg, H., German, J. B., J. Sci. Food Agric. 1996, 70, 55–61.
[40] Clifford, A. J., Ebeler, S. E., Ebeler, J. D., Bills, N. D. et al., Am. J. Clin. Nutr. 1996, 64, 748–756.
[41] Schellinger, A. P., Stoll, D. R., Carr, P. W., J. Chromatogr. A 2005, 1064, 143–156.
[42] Stoll, D. R., Li, X., Wang, X., Carr, P. W. et al., J. Chromatogr. A 2007, 1168, 3–43.
[43] Neue, U. D., J. Chromatogr. A 2005, 1079, 153–161.
[44] Liu, Z., Patterson, D. G., Lee, M. L., Anal. Chem. 1995, 67, 3840–3845.
[45] Dugo, P., Herrero, M., Kumm, T., Giuffrida, D. et al., J. Chromatogr. A 2008, 1189, 196–206.

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Volume31, Issue19

Special Issue: Comprehensive chromatography methods

October 2008

Pages 3297-3308

Use of partially porous column as second dimension in comprehensive two-dimensional system for analysis of polyphenolic antioxidants

Abstract

References

Citing Literature

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Use of partially porous column as second dimension in comprehensive two-dimensional system for analysis of polyphenolic antioxidants

Abstract

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