Volume 46, Issue 6 p. 568-571
Application Note

High spatial resolution imaging mass spectrometry and classical histology on a single tissue section

Fabian Deutskens

Fabian Deutskens

Department of Biochemistry, Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA

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Junhai Yang

Junhai Yang

Department of Biochemistry, Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA

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Richard M. Caprioli

Corresponding Author

Richard M. Caprioli

Department of Biochemistry, Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA

Department of Biochemistry, Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA.===Search for more papers by this author
First published: 01 June 2011
Citations: 88

Abstract

The work presented in this report describes and demonstrates a protocol for protein imaging analysis of biological tissue using MALDI IMS where histological staining and MS analysis are performed on the same tissue section. Spatial image resolution is shown at 35 µm for sagittal sections of the cerebellum from rat brain. Copyright © 2011 John Wiley & Sons, Ltd.