Micromorphological differentiation of Korean Disporum species using light and scanning electron microscopy
Hyeonjin Kim
Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Republic of Korea
Search for more papers by this authorBokyung Choi
Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Republic of Korea
Search for more papers by this authorChangyoung Lee
International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea
Search for more papers by this authorScott A. M. McAdam
Department of Botany and Plant Pathology, Purdue Center for Plant Biology, Purdue University, West Lafayette, Indiana, USA
Search for more papers by this authorJin-Hyub Paik
International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea
Search for more papers by this authorCorresponding Author
Tae-Soo Jang
Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Republic of Korea
Correspondence
Tae-Soo Jang, Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea.
Email: [email protected]
Search for more papers by this authorHyeonjin Kim
Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Republic of Korea
Search for more papers by this authorBokyung Choi
Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Republic of Korea
Search for more papers by this authorChangyoung Lee
International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea
Search for more papers by this authorScott A. M. McAdam
Department of Botany and Plant Pathology, Purdue Center for Plant Biology, Purdue University, West Lafayette, Indiana, USA
Search for more papers by this authorJin-Hyub Paik
International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea
Search for more papers by this authorCorresponding Author
Tae-Soo Jang
Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Republic of Korea
Correspondence
Tae-Soo Jang, Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea.
Email: [email protected]
Search for more papers by this authorReview Editor: Paul Verkade
Abstract
The genus Disporum Salisb. is widely distributed in East Asia, yet phylogenetically relevant morphological traits useful for differentiating many of the small, perennial, herbaceous species remain poorly described. To address this, leaf, floral, pollen, and orbicule micromorphology of four Korean Disporum species was investigated using light and scanning electron microscopy. All Korean Disporum species examined had hypostomatic leaves, with anomocytic stomatal complexes found only on the abaxial epidermis. Guard cell length varied among species, ranging from 44.30 μm in D. viridescens to 53.49 μm in D. uniflorum. The epidermal cells of the investigated Disporum taxa had sinuate anticlinal cell walls on both adaxial and abaxial surfaces. The surface of the guard and subsidiary cells were either smooth with weak striations or had strongly wrinkled striations. The pollen grains of all Korean Disporum taxa were monads, monosulcate with granular aperture membranes, subprolate to prolate in shape with microreticulate or verrucate exine surfaces. The mean size of pollen grains ranged from 46.38 to 49.92 μm in polar length and from 34.39 to 39.58 μm in equatorial diameter across species. Sexine ornamentation was a taxonomically relevant trait for differentiating Korean Disporum taxa. Additionally, the presence of orbicules as well as the orbicular characters (e.g., size, shape, ornamentation, and association pattern) are described for the first time in species from this genus. The present investigation of leaf and floral micromorphology using light and scanning electron microscopy provides valuable information for the taxonomic differentiation and identification of Disporum species in Korea.
Research Highlights
- A detailed micromorphological description of leaf, floral characters (tepal, stigma, style), pollen and orbicule is provided for Korean Disporum species using scanning electron microscopy (SEM) and light microscopy (LM).
- The presence of orbicules and their taxonomic implications in Korean Disporum species are described for the first time.
- Phylogenetically informative pollen and orbicule micromorphological characters are described, improving understanding the systematic relationships of Korean species in the genus Disproum.
CONFLICT OF INTEREST
The authors declare no conflicts of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data in this study are all provided in the figures and the part of results. Any more requests about the original data are available from the corresponding author.
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