Toxicological evaluation of protein powder derived from Cupriavidus necator
Corresponding Author
Vickie Modica
AIBMR Life Sciences, Inc., 1425 Broadway, Suite 458, Seattle, Washington, 98122 USA
Correspondence
Vickie Modica, AIBMR Life Sciences, Inc., 1425 Broadway, Suite 458, Seattle, WA 98122, USA.
Email: [email protected]
Search for more papers by this authorRóbert Glávits
Toxi-Coop Zrt., Berlini utca 47–49, H-1045, Budapest, Hungary
Search for more papers by this authorTimothy S. Murbach
AIBMR Life Sciences, Inc., 1425 Broadway, Suite 458, Seattle, Washington, 98122 USA
Search for more papers by this authorJohn R. Endres
AIBMR Life Sciences, Inc., 1425 Broadway, Suite 458, Seattle, Washington, 98122 USA
Search for more papers by this authorGábor Hirka
Toxi-Coop Zrt., Berlini utca 47–49, H-1045, Budapest, Hungary
Toxi-Coop Zrt., Arácsi út 97, 8230, Balatonfüred, Hungary
Search for more papers by this authorAdél Vértesi
Toxi-Coop Zrt., Arácsi út 97, 8230, Balatonfüred, Hungary
Search for more papers by this authorErzsébet Béres
Toxi-Coop Zrt., Arácsi út 97, 8230, Balatonfüred, Hungary
Search for more papers by this authorIlona Pasics Szakonyiné
Toxi-Coop Zrt., Berlini utca 47–49, H-1045, Budapest, Hungary
Search for more papers by this authorCorresponding Author
Vickie Modica
AIBMR Life Sciences, Inc., 1425 Broadway, Suite 458, Seattle, Washington, 98122 USA
Correspondence
Vickie Modica, AIBMR Life Sciences, Inc., 1425 Broadway, Suite 458, Seattle, WA 98122, USA.
Email: [email protected]
Search for more papers by this authorRóbert Glávits
Toxi-Coop Zrt., Berlini utca 47–49, H-1045, Budapest, Hungary
Search for more papers by this authorTimothy S. Murbach
AIBMR Life Sciences, Inc., 1425 Broadway, Suite 458, Seattle, Washington, 98122 USA
Search for more papers by this authorJohn R. Endres
AIBMR Life Sciences, Inc., 1425 Broadway, Suite 458, Seattle, Washington, 98122 USA
Search for more papers by this authorGábor Hirka
Toxi-Coop Zrt., Berlini utca 47–49, H-1045, Budapest, Hungary
Toxi-Coop Zrt., Arácsi út 97, 8230, Balatonfüred, Hungary
Search for more papers by this authorAdél Vértesi
Toxi-Coop Zrt., Arácsi út 97, 8230, Balatonfüred, Hungary
Search for more papers by this authorErzsébet Béres
Toxi-Coop Zrt., Arácsi út 97, 8230, Balatonfüred, Hungary
Search for more papers by this authorIlona Pasics Szakonyiné
Toxi-Coop Zrt., Berlini utca 47–49, H-1045, Budapest, Hungary
Search for more papers by this authorAdél Vértesi, Erzsébet Béres, and Ilona Pasics Szakonyiné are co-senior authors.
Funding information: The authors disclose that financial support for the research described herein was provided by Kiverdi, Inc., Pleasanton, CA.
Abstract
Microorganisms have the potential to produce nutrient-rich products that can be consumed as food or feed. The protein-rich powder derived from heat treatment of the whole-cell biomass of polyhydroxybutyrate-deficient Cupriavidus necator, a metabolically versatile organism that uses elements found in the air, is an example of such a product. To assess the safety of the protein powder for use as a nutritional ingredient in human food, in accordance with internationally accepted standards, its genotoxic potential and repeated-dose oral toxicity were investigated. A bacterial reverse mutation test, an in vitro mammalian chromosomal aberration test, and an in vivo mammalian micronucleus test were performed. No evidence of mutagenicity or genotoxicity was found. Additionally, a 90-day repeated-dose oral toxicity study in rats was completed, in which a total of 100 male and female Wistar rats were exposed by gavage to daily doses of 1000, 2000, or 3000 mg/kg bw/day of the test material. Following 90 days of continuous exposure, no mortality or treatment-related adverse effects were observed and no target organs were identified. Therefore, a no observed adverse effect level was determined at 3000 mg/kg bw/day, the highest dose tested.
CONFLICT OF INTEREST
The authors have no conflict of interest to report.
Open Research
DATA AVAILABILITY STATEMENT
The data sets generated during these studies are available in the Supporting Information.
Supporting Information
Filename | Description |
---|---|
jat4432-sup-0001-Table S1_Clin Obs Weekly_2022-Sep-28.xlsExcel spreadsheet, 27.5 KB |
Table S1. Summary Weekly Clinical Observations (outside home cage) |
jat4432-sup-0002-Table S2_Clin Obs Daily_2022-Sep-28.xlsExcel spreadsheet, 26.5 KB |
Table S2. Summary Daily Clinical Observations (cage side) |
jat4432-sup-0003-Table S3_Func Obs Battery_2022-Sep-28.xlsExcel spreadsheet, 34 KB |
Table S3. Summary of functional observations (Mean scores/group) |
jat4432-sup-0004-Table S4_Food Consumption_2022-Sep-28.xlsExcel spreadsheet, 35.5 KB |
Table S4. Daily mean food consumption (g/animal/day) |
jat4432-sup-0005-Table S5_Hematology_2022-Sep-28.xlsExcel spreadsheet, 35 KB |
Table S5. Hematology |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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