Volume 41, Issue 2 p. 291-302
RESEARCH ARTICLE

In vitro copper oxide nanoparticle toxicity on intestinal barrier

Alessia Bertero

Alessia Bertero

Department of Environmental Science and Policy (ESP), Università degli Studi di Milano, Milan, Italy

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Graziano Colombo

Graziano Colombo

Department of Biosciences (Department of Excellence 2018-2022), Università degli Studi di Milano, Milan, Italy

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Cristina Cortinovis

Cristina Cortinovis

Department of Health, Animal Science and Food Safety (VESPA), Università degli Studi di Milano, Milan, Italy

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Virginia Bassi

Virginia Bassi

Department of Veterinary Medicine (DIMEVET), Università degli Studi di Milano, Milan, Italy

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Elisa Moschini

Elisa Moschini

Department of Earth and Environmental Sciences, Research Center POLARIS, Università degli Studi di Milano, Bicocca, Milan, Italy

Department of Environmental Research and Innovation, Luxembourg Institute of Science and Technology, Belvaux, Grand Duchy of Luxembourg

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Nicholas Bellitto

Nicholas Bellitto

Department of Veterinary Medicine (DIMEVET), Università degli Studi di Milano, Milan, Italy

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Maria Chiara Perego

Maria Chiara Perego

Department of Veterinary Medicine (DIMEVET), Università degli Studi di Milano, Milan, Italy

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Marco Albonico

Marco Albonico

Department of Veterinary Medicine (DIMEVET), Università degli Studi di Milano, Milan, Italy

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Emanuela Astori

Emanuela Astori

Department of Biosciences (Department of Excellence 2018-2022), Università degli Studi di Milano, Milan, Italy

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Isabella Dalle-Donne

Isabella Dalle-Donne

Department of Biosciences (Department of Excellence 2018-2022), Università degli Studi di Milano, Milan, Italy

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Aharon Gedanken

Aharon Gedanken

Department of Chemistry and Institute for Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel

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Ilana Perelshtein

Ilana Perelshtein

Department of Chemistry and Institute for Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel

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Paride Mantecca

Corresponding Author

Paride Mantecca

Department of Earth and Environmental Sciences, Research Center POLARIS, Università degli Studi di Milano, Bicocca, Milan, Italy

Correspondence

Paride Mantecca, Department of Earth and Environmental Sciences, Research Center POLARIS, Università degli Studi di Milano, Bicocca, 1, Piazza della Scienza, I-20126, Milan, Italy.

Email: [email protected]

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Francesca Caloni

Francesca Caloni

Department of Environmental Science and Policy (ESP), Università degli Studi di Milano, Milan, Italy

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First published: 27 October 2020
Citations: 4

Abstract

The use of CuO nanoparticles (NPs) has increased greatly and their potential effects on human health need to be investigated. Differentiated Caco-2 cells were treated from the apical (Ap) and the basolateral (Bl) compartment with different concentrations (0, 10, 50 and 100 μg/mL) of commercial or sonochemically synthesized (sono) CuO NPs. Sono NPs were prepared in ethanol (CuOe) or in water (CuOw), obtaining CuO NPs differing in size and shape. The effects on the Caco-2 cell barrier were assessed via transepithelial electrical resistance (TEER) evaluation just before and after 1, 2 and 24 hours of exposure and through the analysis of cytokine release and biomarkers of oxidative damage to proteins after 24 hours. Sono CuOe and CuOw NPs induced a TEER decrease with a dose-dependent pattern after Bl exposure. Conversely, TEER values were not affected by the Ap exposure to commercial CuO NPs and, concerning the Bl exposure, only the lowest concentration tested (10 μg/mL) caused a TEER decrease after 24 hours of exposure. An increased release of interleukin-8 was induced by sono CuO NPs after the Ap exposure to 100 μg/mL and by sono and commercial CuO after the Bl exposure to all the concentrations. No effects of commercial and sono CuO NPs on interleukin-6 (with the only exception of 100 μg/mL Bl commercial CuO) and tumor necrosis factor-α release were observed. Ap treatment with commercial and CuOw NPs was able to induce significant alterations on specific biomarkers of protein oxidative damage (protein sulfhydryl group oxidation and protein carbonylation).

CONFLICT OF INTEREST

The authors have no conflict of interest to report.