Volume 27, Issue 16 p. 3306-3321
Proteomics and 2-DE

A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis

Jean-Paul Lasserre

Jean-Paul Lasserre

Plateforme Génomique Fonctionnelle Bordeaux – Pôle Protéomique, Université Victor Segalen Bordeaux 2, Bordeaux, France

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Emmanuelle Beyne

Emmanuelle Beyne

Laboratoire Bordelais de Recherche en Informatique, Domaine Universitaire, Talence, France

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Slovénie Pyndiah

Slovénie Pyndiah

EA 516 Bacteriologie et épidémiologie des infections digestives, Université Victor Segalen Bordeaux 2, Bordeaux, France

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Delphine Lapaillerie

Delphine Lapaillerie

Plateforme Génomique Fonctionnelle Bordeaux – Pôle Protéomique, Université Victor Segalen Bordeaux 2, Bordeaux, France

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Stéphane Claverol

Stéphane Claverol

Plateforme Génomique Fonctionnelle Bordeaux – Pôle Protéomique, Université Victor Segalen Bordeaux 2, Bordeaux, France

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Marc Bonneu Professor

Corresponding Author

Marc Bonneu Professor

Plateforme Génomique Fonctionnelle Bordeaux – Pôle Protéomique, Université Victor Segalen Bordeaux 2, Bordeaux, France

Plateforme Génomique Fonctionnelle Bordeaux – Pôle Protéomique, Université Victor Segalen Bordeaux 2, 146, rue Léo Saignat, F-33076 Bordeaux, France Fax: +33-5-5757-1684===Search for more papers by this author
First published: 16 August 2006
Citations: 94

Abstract

Study of the complexome – all the protein complexes of the cell – is essential for a better understanding and more global vision of cell function. Using two-dimensional blue native/SDS-PAGE (2-D BN/SDS-PAGE) technology, the cytosolic and membrane protein complexes of Escherichia coli were separated. Then, the different partners of each protein complex were identified by LC-MS/MS. In this report, 306 protein complexes were separated and identified. Among these protein complexes, 50 heteromultimeric and 256 homomultimeric protein complexes were found. Among the 50 heteromultimeric protein complexes, 18 previously described protein complexes validate the technology. In this study, 109 new protein complexes were found, providing insight into the function of previously uncharacterized bacterial proteins.