Volume 35, Issue 1 e202100672
Research Article

Electrochemical Immunosensor Made with Zein-based Nanofibers for On-site Detection of Aflatoxin B1

Danilo M. Dos Santos

Corresponding Author

Danilo M. Dos Santos

Nanotechnology National Laboratory for Agriculture (LNNA), Embrapa Instrumentação, 13560-970 São Carlos, SP, Brazil

These authors contributed equally to this work.

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Fernanda L. Migliorini

Fernanda L. Migliorini

Nanotechnology National Laboratory for Agriculture (LNNA), Embrapa Instrumentação, 13560-970 São Carlos, SP, Brazil

These authors contributed equally to this work.

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Andrey C. Soares

Andrey C. Soares

Nanotechnology National Laboratory for Agriculture (LNNA), Embrapa Instrumentação, 13560-970 São Carlos, SP, Brazil

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Luiz H. C. Mattoso

Luiz H. C. Mattoso

Nanotechnology National Laboratory for Agriculture (LNNA), Embrapa Instrumentação, 13560-970 São Carlos, SP, Brazil

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Osvaldo N. Oliveira Jr.

Osvaldo N. Oliveira Jr.

São Carlos Institute of Physics, University of São Paulo, P.O. Box 369, São Carlos, 13560-970, SP Brazil

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Daniel S. Correa

Corresponding Author

Daniel S. Correa

Nanotechnology National Laboratory for Agriculture (LNNA), Embrapa Instrumentação, 13560-970 São Carlos, SP, Brazil

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First published: 17 May 2022
Citations: 3

Abstract

A disposable electrochemical immunosensor for on-site detection of aflatoxin B1(AFB1), one of the most toxic mycotoxins in agri-food products, was fabricated through a low-cost cut-printing method and then modified with zein/polypyrrole(PPy) electrospun nanofibers onto which anti-AFB1 monoclonal antibodies were immobilized covalently. Fabrication was possible with an innovative and simple approach to adsorb nanofibers onto the working electrode during electrospinning. Electrochemical impedance spectroscopy was employed as the principle of detection, and the data collected with a portable potentiostat were treated with information visualization techniques. The nanostructured immunosensor showed a high sensitivity for AFB1 with a linear detection range from 0.25 to 10 ng mL−1 and a theoretical limit of detection of 0.092 ng mL−1, which is adequate to detect AFB1 in food, according to regulatory agencies.

Data Availability Statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.