Volume 34, Issue 7 e4831
RESEARCH ARTICLE

Bias reduction in the quantitative analysis of a target analyte present in a limited quantity in human plasma using dual-mode heart-cutting two-dimensional liquid chromatography coupled with isotope dilution mass spectrometry

Seok-Won Hyung

Corresponding Author

Seok-Won Hyung

Division of Chemical and Medical Metrology, Korea Research Institute of Standards and Science, Daejeon, South Korea

Correspondence

Seok-Won Hyung, Division of Chemical and Medical Metrology, Korea Research Institute of Standards and Science, Yuseong, Daejeon 34113, South Korea.

Email: [email protected]

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Byungjoo Kim

Byungjoo Kim

Division of Chemical and Medical Metrology, Korea Research Institute of Standards and Science, Daejeon, South Korea

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First published: 17 March 2020
Citations: 2

Abstract

Dual-mode heart-cutting two-dimensional liquid chromatography (DMHC 2D-LC) was applied to isotope dilution mass spectrometry (IDMS) to reduce the bias in the quantitative analysis of a target analyte present in a limited quantity in human plasma. Based on a Waters I-Class LC system, the DMHC 2D-LC system was operated in one- and two-dimensional modes to facilitate the determination of heart-cutting time and the efficient trapping of the target LC eluate. Experiments to determine the feasibility of coupling with IDMS were performed with triple quadrupole mass spectrometry using folic acid standards and/or 13C5-folic acid. To validate the performance of the DMHC 2D-LC/IDMS system on a complex sample, human plasma was analyzed for folic acid and the result was compared with that obtained using conventional single-column LC. The total run time of the DMHC 2D-LC system was 20 min, the same as that of the single-column LC system. The peak profile of the spiked 13C5-folic acid obtained with single-column LC/MS was affected by matrix effects, but resolved with DMHC 2D-LC/MS, thus improving the accuracy of the analysis. The DMHC 2D-LC/IDMS system showed reliable performance in analyzing the target analyte in human plasma, eliminating matrix effects and saving analysis time.