Volume 23, Issue 3 p. 273-279
Research Article
Free Access

Method validation for measurement of hair nicotine level in nonsmokers

Sung Roul Kim

Corresponding Author

Sung Roul Kim

The Institute for Global Tobacco Control, Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA

615 N. Wolf Street W7010H, Baltimore, MD 21205, USA.Search for more papers by this author
Heather Wipfli

Heather Wipfli

The Institute for Global Tobacco Control, Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA

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Erika Avila-Tang

Erika Avila-Tang

The Institute for Global Tobacco Control, Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA

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Jonathan M. Samet

Jonathan M. Samet

The Institute for Global Tobacco Control, Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA

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Patrick N. Breysse

Patrick N. Breysse

Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA

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First published: 23 September 2008
Citations: 36

Abstract

The development of strategies to address the growing worldwide burden of exposure to secondhand smoke (SHS) would be facilitated by sensitive and accurate methods for assessing SHS exposure. Hair provides a readily available matrix for assessing biomarkers of typical SHS exposure. We developed and applied an optimized analytical method using an isotope dilution gas chromatography–mass spectrometry (GC/MS) for hair nicotine measurement. The utility of this optimized method is illustrated by presenting data on SHS exposure of women and children from 31 countries. Using this isotope dilution method with spiked samples (3.3 ng/mg), we found that the greatest hair nicotine extraction efficiency was obtained with a 60 min shaking time. In the field study (n = 2400), a positive association was evident between hair nicotine concentrations from nonsmokers and higher numbers of cigarettes smoked per day in a household. Copyright © 2008 John Wiley & Sons, Ltd.

Abbreviations used

  • GC/MS
  • gas chromatography–mass spectrometry

  • LOD
  • limit of detection

  • SHS
  • secondhand smoke