Volume 19, Issue 2 2300390
RESEARCH ARTICLE

A generic pump-free organ-on-a-chip platform for assessment of intestinal drug absorption

Yaqiong Guo

Yaqiong Guo

Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China

Contribution: Conceptualization (lead), Methodology (lead), Project administration (equal), Visualization (equal), Writing - review & editing (lead)

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Yingying Xie

Yingying Xie

Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China

University of Chinese Academy of Sciences, Beijing, China

Contribution: Data curation (lead), ​Investigation (lead), Project administration (equal), Visualization (equal), Writing - original draft (lead)

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Jianhua Qin

Corresponding Author

Jianhua Qin

Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China

University of Chinese Academy of Sciences, Beijing, China

Beijing Institute for Stem Cell and Regenerative Medicine, Chinese Academy of Sciences, Beijing, China

University of Science and Technology of China, Hefei, China

Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou, China

Correspondence

Jianhua Qin, Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Email: [email protected]

Contribution: Funding acquisition (lead), Supervision (lead), Writing - review & editing (supporting)

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First published: 20 February 2024

Yaqiong Guo and Yingying Xie contributed equally to this work.

Abstract

Organ-on-a-chip technology has shown great potential in disease modeling and drug evaluation. However, traditional organ-on-a-chip devices are mostly pump-dependent with low throughput, which makes it difficult to leverage their advantages. In this study, we have developed a generic, pump-free organ-on-a-chip platform consisting of a 32-unit chip and an adjustable rocker, facilitating high-throughput dynamic cell culture with straightforward operation. By utilizing the rocker to induce periodic fluid forces, we can achieve fluidic conditions similar to those obtained with traditional pump-based systems. Through constructing a gut-on-a-chip model, we observed remarkable enhancements in the expression of barrier-associated proteins and the spatial distribution of differentiated intestinal cells compared to static culture. Furthermore, RNA sequencing analysis unveiled enriched pathways associated with cell proliferation, lipid transport, and drug metabolism, indicating the ability of the platform to mimic critical physiological processes. Additionally, we tested seven drugs that represent a range of high, medium, and low in vivo permeability using this model and found a strong correlation between their Papp values and human Fa, demonstrating the capability of this model for drug absorption evaluation. Our findings highlight the potential of this pump-free organ-on-a-chip platform as a valuable tool for advancing drug development and enabling personalized medicine.

CONFLICT OF INTEREST STATEMENT

The authors declare no commercial or financial conflict of interest.

DATA AVAILABILITY STATEMENT

The data that support the findings of this study is available from the corresponding author upon reasonable request.